Peer-Reviewed Publication
Genome Biol Evol2026September 7, 2026Journal Article

A chromosome-scale assembly for the genome of southern corn rootworm, Diabrotica undecimpunctata.

Brad S Coates1, Scott M Geib2, Luke R Tembrock3,4, Ana Velez5, Monica Poelchau6, Christopher Childers6, Ana Childers7, Amanda Cooksey8, Kevin Hackett9, Sheina B Sim2
1USDA, Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, 819 Wallace Road, Ames, IA 50011.
2USDA, Agricultural Research Service, U.S. Pacific Basin Agricultural Research Center, Tropical Pest Genetics and Molecular Biology Research Unit, 64 Nowelo Street, Hilo, Hawaii 96720.
3USDA, Animal and Plant Health Inspection Service, Plant Protection and Quarantine, Science & Technology, 2301 Research Boulevard, Fort Collins, CO 80526, USA.
4Department of Agricultural Biology, Colorado State University, Fort Collins, CO 80523, USA.
5Department of Entomology, University of Nebraska, Lincoln, NE 68583-0816  USA.
6USDA, National Agricultural Library, Beltsville, MD 20705  USA.
7USDA, Agricultural Research Service, Bee Research Laboratory, Beltsville, Maryland 20705  USA.
8Department of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA.
9USDA, Agricultural Research Service, Office of National Programs, Beltsville, MD 20705  USA.

Abstract

Diabrotica undecimpunctata ssp. howardi, the southern corn rootworm or eastern 12-spotted cucumber beetle, is a generalist insect herbivore that causes damage and yield loss to several crops in North America including maize. Unresolved phylogenetic relationships within and among D. undecimpunctata subspecies are impacting current quarantine policies. We report the chromosome-level haploid genome a…

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