Establishment and application of a multiplex qPCR panel for multi-host surveillance of tick-Borne pathogens.

Jing Li¹, Zhen Wang¹, Jing Li², Xinting Meng¹, Jiaxun Zhang¹, Wenjin Mao¹, Yan Hai³, Ying Zhang⁴, Xiangxiang Sun⁵, Jin Che⁶, Xiaofang Ma⁷, Biao Duan⁸, Aiping Qin⁹, Jie Liu¹

¹School of Public Health, Qingdao University, Shandong, People's Republic of China.

²Department of Laboratory Medicine, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.

³Department of Biosafety, General Center for Disease Control and Prevention of Inner Mongolia Autonomous Region, Hohhot City, People's Republic of China.

⁴Department of Vector-Borne Disease, Center for Disease Control and Prevention of Xilingol League, Xilinhaote, People's Republic of China.

⁵Key Laboratory of Animal Tuberculosis, Ministry of Agriculture and Rural Affairs (Reference Laboratory for Animal Tuberculosis), China Animal Health and Epidemiology Center, Qingdao, People's Republic of China.

⁶State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research Institute, Gansu, People's Republic of China.

⁷Institute of Disinfection and Vector Control, Qingdao Municipal Center for Disease Control and Prevention, Qingdao, People's Republic of China.

⁸Plague Prevention and Control Division, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, People's Republic of China.

⁹Department of Respiratory Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

PubMed DOI

Abstract

Tick-borne diseases pose significant public health threats. Non-specific symptoms and co-infections present diagnostic challenges for conventional methods. To address this, we developed the tick-borne pathogen (TBP) Panel, utilizing eight separate multiplex qPCR assays to simultaneously detect 25 TBPs (7 bacteria, 14 viruses, 4 parasites). Optimal Quantification Cycle (Cq) cut-offs were establishe…

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