A protocol for ploidy determination in ethanol-fixed Atlantic salmon (Salmo salar) fry using propidium iodide and internal standardization: Compatible with field conditions at the sample preservation stage.

Sebastián Ávila¹, Maira Toro², Wellison Amorim Pereira³, Christian Ellwanger-Reyes⁴, Iván Valdebenito Isler⁴, Elías Figueroa-Villalobos⁵

¹Doctorado en Ciencias Agropecuarias, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile.

²Facultad de Ciencias Agropecuarias y Medio Ambiente, Universidad de La Frontera, Temuco, Chile.

³Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

⁴Departamento de Ciencias Agropecuarias y Acuícolas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile.

⁵Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile.

PubMed PMC DOI PDF

Abstract

Ploidy verification in salmonid production is often constrained by the need to analyze fresh samples. Here, we present an optimized protocol for early-stage ploidy determination in Atlantic salmon (Salmo salar) fry using 70 % ethanol fixation at -20 °C and flow cytometry. A staining solution containing Propidium Iodide (PI) and RNase A was employed to ensure stoichiometric DNA quantification, comb…

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